Package: BKMutate 0.1.0

BKMutate: Statistical Analysis of Induced Mutagenesis Experiments in Crop Plants

A colour-first toolkit for the statistical analysis of induced mutagenesis experiments in crop plants. It fits dose-response models to physical and chemical mutagen data and estimates the median lethal and growth-reduction doses (LD50, GR50) with confidence intervals obtained from Fieller's theorem; quantifies first-generation biological damage (lethality, injury and pollen sterility); and estimates mutagenic effectiveness and mutagenic efficiency. Effectiveness and efficiency are conventionally reported as point estimates only; this package treats them as functions of binomial proportions and supplies interval estimates by the delta method on the logarithmic scale and by the nonparametric bootstrap. It further provides chlorophyll mutation spectrum analysis with tests of homogeneity and diversity, generalised linear models for second-generation mutant counts with formal assessment of overdispersion, and formal comparison of mutagens including relative biological effectiveness. Every analysis returns a tidy result object and a publication-ready 'ggplot2' figure. Methods follow Konzak et al. (1965, ISBN:9789201150653), Fieller (1954) <doi:10.1111/j.2517-6161.1954.tb00159.x> and Katz et al. (1978) <doi:10.2307/2530610>.

Authors:Praveen Kumar B. K. [aut, cre]